ONETest™ Platform

Targeted metagenomics, built for hospital labs.

ONETest™ integrates QuantumProbes™ core-genome capture, UniPrep™ library chemistry, walk-away automation, sequencing, and FusionCloud™ / Fusion AI into one RUO platform supporting accredited-lab metagenomic workflows.

ONETest™ reagents and software are provided for research use only. Clinical use requires independent validation, performance, and reporting by an appropriately accredited laboratory under its applicable regulatory, accreditation, and quality framework.

View Workflow Hospital Deployment

Workflow

Implementation

Technology

Integrated platform stack

QuantumProbes™

Core-genome capture across pathogens and related lineages

UniPrep™ + automation

Standardized DNA-to-sequencer workflow for hospital labs

FusionCloud™ / Fusion AI

Actionable Intelligence for evidence interpretation

Workflow

From sample to actionable report.

The ONETest™ workflow is designed to make targeted metagenomics operationally practical in a hospital environment: capture the right microbial DNA, prepare a sequencing-ready library, automate repeatable steps, and return ranked analytical outputs through FusionCloud™.

BAL / laboratory sample

Specimen enters a standardized molecular workflow.

QuantumProbes™ capture

Core-genome regions are enriched across target organisms and related lineages.

UniPrep™ library

Captured DNA is converted into a sequencing-ready DNA library.

Automation + sequencing

Liquid-handling automation supports reproducible DNA-to-sequencer processing.

FusionCloud™ analysis

Fusion AI applies background-aware signal analysis and ranked outputs.

Actionable report

Results are structured for interpretation within a <24-hour workflow.

Implementation

Designed for on-site implementation & validation, not just sequencing performance.

ONETest™ is built around the practical requirements that determine laboratory implementation: automation, standardized reagents, in-lab sequencing compatibility, cloud-based interpretation, and an implementation path aligned with accredited laboratory validation workflows.

<24h

Sample-to-result target

Built around timely laboratory workflows for complex infection evaluation.

LDT

Accredited-lab pathway

Designed to support validation within accredited laboratory workflows.

Hospital Lab Fit

Designed for lab integration

ONETest™ is designed to run with standard liquid-handling automation and desktop sequencing infrastructure supporting in-house workflows where validation and infrastructure are in place.

FusionCloud™ / Fusion AI

The interpretation layer

FusionCloud™ transforms sequence outputs into ranked, interpretable results using background-aware signal qualification and organism-specific thresholds where validated.

Deployment Playbook

Validate and replicate across networks.

The implementation model links clinical validation, IT/security readiness, automation, and reporting into a repeatable site-validation package.

Technical Engine

A new class of targeted metagenomics.

ONETest™ is not built around a fixed list of short amplicons or a brute-force whole-metagenome workflow. It uses QuantumProbes™ to enrich conserved microbial core-genome regions, preserving meaningful microbial community structure while increasing the pathogen signal needed for clinical interpretation.

Core-Genome Capture

QuantumProbes™ target hunderds of thousands of core-genomes from 50 microbial families, enabling broad organism detection and species-level resolution.

Microbiome Representation

ONETest™ preserves a representative view of the microbial community, with approximately R² = 0.74 concordance to whole-metagenome sequencing in BAL samples.

Clinical Signal Enrichment

By increasing on-target microbial abundance, ONETest™ reduces sequencing burden and creates cleaner inputs for FusionCloud™ AI interpretation.

QuantumProbes™

Core-genome enrichment at diagnostic scale.

QuantumProbes™ can capture up to 20% in genetic distance allowing them to bind all related lineages, thus achieving the capture profile needed for microbiome interpretation.

6.2M

QuantumProbes™

Microbial families

250+

Respiratory pathogens

26×

On-target enrichment

Evidence

Representative microbiome profiling.

ONETest™ preserves within-sample microbial diversity while improving sequencing efficiency. The correct manuscript-supported comparison is Shannon diversity correlation of r = 0.74 versus WmGS across BAL samples.

r = 0.74

Shannon diversity correlation vs. WmGS

Shannon diversity comparison showing preservation of within-sample microbial diversity.

Targeted enrichment improves microbial signal while reducing inefficient sequencing burden.

Validation Snapshot

Evidence from laboratory validation.

The ONETest™ BAL evaluation supports the platform as a complementary laboratory approach for complex lower-respiratory evaluation workflows, including culture-limited and polymicrobial presentations.

99%

Species-level specificity

High specificity with control-informed interpretation in the BAL clinical cohort.

80%

Species-level sensitivity

Across culture-confirmed isolates in the BAL clinical cohort.

21%

Additive yield

Additional BAL findings beyond routine bacterial / AFB culture; requires clinical correlation.

85%

Lab-specific LDT validation

Detected ≥1 culture-confirmed organism in culture-positive validation specimens.

From Dx To Rx in <24 Hours

Targeted metagenomics, automation, and Actionable Intelligence in one platform.

ONETest™ helps reduce the gap between sample and structured microbiologic evidence by transforming genomic data into ranked clinical intelligence for hospital laboratories and critical-care teams.

Contact Fusion Genomics