At Fusion Genomics, our legacy portfolio showcases how QuantumProbes™ targeted metagenomics supported respiratory-virus detection, SARS-CoV-2 genome sequencing, environmental surveillance, and One Health applications.
These programs helped establish the platform foundation for today’s ONETest™ PathoGenome and FusionCloud™ Actionable Intelligence strategy.
Toronto clinical respiratory specimens evaluated against conventional testing.
UF SARS-CoV-2-positive respiratory samples processed for genome sequencing.
Wetland and farm sediment samples analyzed for avian influenza surveillance.
Respiratory-virus groups represented in the legacy viral assay portfolio.
Historical studies and materials are summarized for platform context. Current product availability and use remain subject to applicable laboratory validation and regulatory requirements.
The legacy respiratory and surveillance assays demonstrated a central Fusion principle: targeted capture can enrich informative microbial signal while retaining sequence-level context for typing, lineage assignment, and surveillance.
Probe-based capture focused sequencing on informative viral loci and reduced dependence on deep whole-metagenome sequencing.
Legacy panels covered SARS-CoV-2, seasonal coronaviruses, influenza A/B, RSV, hMPV, parainfluenza, enterovirus/rhinovirus, parechovirus, adenovirus, and bocavirus.
The platform supported subtype, lineage, phylogenetic, and abundance-informed analysis rather than limiting output to a binary detection call.
Clinical swabs, endotracheal aspirates, wetland sediment, farm waterbody sediment, wastewater, and wildlife-related samples informed the platform’s evolution.
The legacy ONETest™ respiratory-virus portfolio was applied across four complementary verticals, each demonstrating a different value of targeted metagenomics.
Clinical respiratory specimens from Toronto hospitals were compared against conventional respiratory-virus assays to evaluate ONETest™ detection and agreement.
ONETest™ Coronaviruses Plus was applied to SARS-CoV-2-positive respiratory samples and compared with ARTIC amplicon sequencing.
Sediment-based targeted resequencing was used to detect and characterize avian influenza virus across wetland and farm-associated samples.
Airport wastewater and wildlife-oriented studies extended the platform into surveillance settings where population-level viral signals can inform public-health awareness.
The Toronto clinical study evaluated ONETest™ as a target-capture next-generation sequencing platform for detecting and identifying medically important respiratory viruses in symptomatic and asymptomatic cohorts.
Across pathogens tested, ONETest™ demonstrated high specificity and strong agreement with conventional respiratory-virus assays, while providing sequence-based evidence, breadth-of-coverage information, and normalized abundance measures.
symptomatic-patient specimens
asymptomatic outpatient specimens
sensitivity range
specificity range
During the COVID-19 era, ONETest™ Coronaviruses Plus extended the legacy respiratory-virus platform into SARS-CoV-2 genome sequencing and wastewater surveillance.
In a published UF Health study, ONETest™ generated complete or near-complete SARS-CoV-2 genomes in 45 of 70 PCR-positive respiratory samples and showed fewer coverage-loss regions than ARTIC in key comparisons.
Fusion’s Toronto airport wastewater work applied ONETest™-based target enrichment and FusionCloud™ analysis to respiratory pathogens and SARS-CoV-2 variant patterns across airport locations and collection dates.
In collaboration-linked programs involving BC public-health, agriculture, and academic partners, ONETest™-based targeted resequencing was applied to wetland and farm sediment samples collected during the 2014–2015 avian influenza outbreak in British Columbia.
These studies demonstrated the feasibility of sediment as a pooled environmental substrate for detecting and characterizing avian influenza virus diversity.
Journal of Wildlife Diseases study: AIV was identified in 71 of 345 wetland and farm sediment samples.
Frontiers study: enrichment-based targeted resequencing identified AIV sequences in 79 of 345 RNA extracts.
Multiple hemagglutinin subtypes were detected, including H5 and other environmentally relevant AIV signals.
As many as eight HA and eight NA subtypes were detected in a single sediment sample.
The legacy portfolio created a technical and operational foundation for later hospital-focused targeted metagenomics programs.
BC Ministry of Agriculture and BCCDC-linked pilot work explored wetland sediment as a surveillance substrate for avian influenza genomic analysis.
Targeted enrichment was assessed for seasonal respiratory-pathogen surveillance, including semi-quantitative FPKM and coverage relationships with Ct values.
Published studies showed targeted resequencing could detect and characterize avian influenza diversity from complex sediment matrices.
ONETest™ Coronaviruses Plus was published as a target-capture approach for SARS-CoV-2 genome sequencing from UF respiratory samples.
The same targeted-metagenomic foundation now supports ONETest™ PathoGenome, focused on hospital-deployable infectious-disease workflows and Actionable Intelligence.
The legacy ONETest™ respiratory-virus programs showed that targeted capture could recover actionable microbial sequence information from challenging sample types. ONETest™ PathoGenome extends this principle into lower-respiratory and critical-care infection workflows, integrating broader pathogen coverage, automation, and FusionCloud™ Actionable Intelligence.
Explore the current targeted metagenomics workflow for hospital laboratories.
See how genomic signal is converted into structured evidence and reporting support.
Contact Fusion Genomics for platform, collaboration, or technical-resource inquiries.
Selected legacy materials can be linked here as public resources or retained as internal supporting documentation.
Diagnostic Microbiology and Infectious Disease, 2021.
Journal of Wildlife Diseases, 2020.
Frontiers in Veterinary Science, 2020.
Targeted metagenomic sequencing for respiratory-pathogen surveillance.
Retrospective assessment of targeted enrichment for respiratory pathogens.
Legacy assay information sheet for respiratory-virus target capture.
The ONETest™ respiratory-virus and environmental-surveillance programs described on this page reflect historical research, development, and collaborative studies conducted by Fusion Genomics and partners. These legacy programs are presented for platform-history and evidence-context purposes.
These materials are not intended to represent currently marketed diagnostic products. ONETest™ reagents and software are supplied for research use only unless independently validated and used by an appropriately accredited laboratory under its applicable regulatory, accreditation, and quality framework.
Targeted metagenomics, automation, and FusionCloud™ interpretation have evolved from legacy respiratory-virus surveillance into hospital-ready workflows for critical-care infection diagnosis.