The ONETest™ Influenza EnviroScreen assay was developed for sensitive and scalable detection of influenza viruses in environmental samples. The assay is highly optimized for soil and wetland sediment samples from temperate regions, and can simultaneously identify and differentiate all 16 hemagglutinin and 9 neuraminidase subtypes. Thus, the assay can facilitate large-scale environmental screening for influenza viruses.
The ONETest™ Influenza EnviroScreen allows:
Sensitive detection of inﬂuenza viruses in environmental samples, especially soil and wetland sediment samples
Simultaneous identiﬁcation of all known inﬂuenza viruses (H1 to H16 and N1 to N9) in a single reaction
Detection of multiple co-occurring types, subtypes, and strainsDiscovery of mutations possibly associated with changes in certain phenotypic traits (e.g., pathogenicity)
Semi-quantitative estimation of viral load.
Figure: Screening environmental samples. Data was generated using an illumina NextSeq machine. Influenza typing was performed on the FUSIONCloud™. The data shows that the assay can identify H5N2 and other co-occurring influenza virus subtypes.