Our ONETest Influenza Sentinel

Why did we develop the ONETest Influenza Sentinel?

Commonly called “The Flu”, the Influenza virus has a major impact on global health. Every year 5-10% of the world’s population is infected with Flu virus which leads to between 3 and 5 million hospitalizations and results in up to half a million deaths.

WHO-Influenza-Specimens-Map

Percentage of Respiratory Specimens That Tested Positive for Influenza Jan 18-24, 2015 (map from the Council on Foreign Relations’ Garrett on Health newsletter)

The best way to treat the Flu is to keep from getting it in the first place. That’s why people are encouraged to get treated with vaccines. They boost the response of our immune systems and help to keep us from getting the Flu. The problem is that there are many different kinds of Flu virus and we need to know which one is infecting people so that we can immunize people against the correct form.

In order to determine which kinds of Flu are present locally and causing outbreaks, healthcare systems undertake surveillance of the population.

Influenza virus comes in 3 types, A, B and C. Influenza C virus causes a mild respiratory illness, is not thought to cause epidemics, and infections usually resolve without treatment.

Influenza A and B viral types are the troublesome ones and can be further subdivided into strains based on 2 proteins found on the surface of the virus, Hemagglutinin and Neuraminidase, usually abbreviated as H and N.

There are 18 different H and 11 different N variants so identifying which one of the subtypes a person is infected with can be quite time consuming.

The current influenza genotype testing standard to identify all these subtypes requires 22 different tests along with pre-NGS Sanger sequencing and is cumbersome, less sensitive, and time consuming.

Description Back to Top

FUSION GENOMICS developed our ONETest Influenza Sentinel assay for the sensitive and scalable identification of all major circulating influenza viruses with one simple test.

The assay provides more information than all widely employed conventional tests combined (i.e., RT-PCR assays and Sanger sequencing), including estimates of viral load and quasi-species. The assay enables various types of epidemiological investigations – from routine surveillance to vaccine effectiveness studies – in a single, simple-to-perform assay.

We put the standard PCR assay used by the WHO to identify Flu strains up against our ONETest Influenza Sentinel assay to see which test captured more of the standard H1 Flu strains.

H1 Quantum Probe Assay vs PCR-new2

Clearly, our ONETest Influenza Sentinel outperformed WHO’s standard protocol RT-PCR by capturing 95% of the H1 strains, more than 2 times as many as the PCR test.

The ONETest Influenza Sentinel also gives you access to our FUSIONCloud™ to upload the raw sequence data and receive the results of your data analysis.

The ONETest Influenza Sentinel assay was applied on a H1N1 influenza virus-positive sample.
Example data demonstrates the assay’s capability to (1) identify influenza type, subtype, and strain
correctly, (2) recover the entire sequences of HA and NA, and (3) mutations and quasi-species.

A

Sentinel A

B

Sentinel B

Example read coverage over the (A) hemagglutinin (HA) and (B) neruaminidase (NA) segments. Data was generated using an Illumina NextSeq machine from a H1N1 influenza virus-positive sample processed using the ONETest Influenza Sentinel assay. Influenza typing and reconstruction of the HA and NA consensus sequences were performed on the FusionCloud. The reads were aligned to the consensus sequences, and their alignments were visualized using IGV1,2.

Sentinel refs

How well does the ONETest Influenza Sentinel fill the need?

The ONETest Influenza Sentinel assay has was been tested and validated by a major reference lab in British Columbia, Canada. The data proved that our ONETest Influenza Sentinel is much more sensitive than existing PCR methods and gives detailed strain-level data.

Applications Back to Top

The more comprehensive data received from the ONETest Influenza Sentinel assay allows:

  • Capture of the entire hemagluttinin (HA) and neuramnidase (NA) gene segments as well as conserved regions of the matrix segment
  • Simultaneous identification of all major ciruclating influenza viruses (A/H1N1, A/H3N2, B/Victoria, and B/Yamagat) in a single reaction
  • Detection of multiple co-occuring types, subtypes and strains
  • Discovery of mutations possibly associated with antigenic drift or virulence as well as quasi-species within a sample
  • Semi-quantitative estimation of viral load

Storage and UsageBack to Top

Store at -20 C. Reactions are conducted on ice.

Testing & DevelopmentBack to Top

Who is currently testing our ONETest Influenza Sentinel?

The ONETest Influenza Sentinel is undergoing a 100 patient pilot test with a major reference center in the Netherlands for 2016 influenza season genotype reporting to the WHO. A successful pilot will lead to additional sales and EU-wide use of the test.

FAQs & Tips Back to Top

  1. Q: How much sample material do I need?

    A: As much as it takes to yield a minimum of 200ng of high quality RNA for library construction.

  2. Q: How much does the kit cost?
  3. A: Please contact us for pricing.

  4. Q: Are there extra costs for analysing my data?

    A: No. Web-based data analysis is included in the kit.

Protocols & Manual Back to Top

Please contact us for protocols and manuals.

Is the ONETest Influenza Sentinel available?

The test is currently available as a Research Use Only product. Please contact us if you would like to obtain this product.

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